Proteolysin, a Novel Highly Thermostable and Cosolvent-Compatible Protease from the Thermophilic Bacterium Coprothermobacter proteolyticus
نویسندگان
چکیده
منابع مشابه
Proteolysin, a novel highly thermostable and cosolvent-compatible protease from the thermophilic bacterium Coprothermobacter proteolyticus.
Through genome mining, we identified a gene encoding a putative serine protease of the thermitase subgroup of subtilases (EC 3.4.21.66) in the thermophilic bacterium Coprothermobacter proteolyticus. The gene was functionally expressed in Escherichia coli, and the enzyme, which we called proteolysin, was purified to near homogeneity from crude cell lysate by a single heat treatment step. Proteol...
متن کاملProteolysin , a Novel Highly Thermostable and Cosolvent - Compatible 1 Protease from the Thermophilic Bacterium
# Corresponding author. Mailing address: Biotransformation and Biocatalysis, 18 Groningen Biomolecular Science and Biotechnology Institute, University of 19 Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. E-mail: 20 [email protected]. Phone: +31-50-3634008; Fax: +31-50-3634165. 21 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. d...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2013
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01479-13